Genetically Encoded Dihydroxyphenylalanine Coupled with Tyrosinase for Strain Promoted Labelling

Abstract

\n Protein modifications through the genetic code engineering method have a remarkable impact on macromolecule engineering, protein translocation, protein-protein interaction, and cell biology. Here, the newly developed molecular biology approach expanding the genetic code was used for fine tuning of protein for biological availability. For that non-canonical amino acid dihydroxyphenylalanine was genetically incorporated at the defined site in the protein. Further, the congener protein was enzymatically controlled for direct conversion of quinone for strain promoted click chemistry reaction. It yields a single product with defined stereochemistry and temporally controlled conjugation with BCN. The feasibility was explored for selective cell imaging and programmed cell death in HeLa cells.