First report of leaf spots caused by Pestalotiopsis trachicarpicola in Gentiana rhodantha

Abstract

\n In August 2018, leaf spots were widely found with circular or irregular brown lesions on Gentiana rhodantha leaves in Liupanshui, Guizhou Province, China. Three strains of Pestalotiopsis spp were associated with leaf lesions by single-spore isolation. Based on asexual and sexual characteristics and multi-locus (ITS, TUB, tef-1 ) phylogenies data, strains were identified as Pestalotiopsis trachicarpicola . This is the first report of G. rhodantha leaf spot caused by P. trachicarpicola . The fungal culture tests showed that mycelia optimum growth temperature and pH were 20–25°C and pH 7–9, and for conidia germination were 25–30°C and pH 6–9, respectively.