Repair Effects of NGF and FPS-ZM1 on brains of epileptic rats

Abstract

\n Background Epilepsy in children seriously affects the quality of life and learning ability of children.And it is very important for repairing the brain function of children after seizure.In this study ,we will investigates the neuroprotective effects of NGF and FPS-ZM1 by measuring HMGB1of hippocampus and serum after intervening the rats with NGF and FPS-ZM1. It would provide theoretical basis for the treatment of brain injury caused by epilepsy. Methods 130 Wistar male young rats were randomly divided into three groups: Group A (normal control group including 31 rats), group B (epilepsy group including 33 rats), group C (NGF group including 33 rats), group D (FPS-ZM1 group including 33 rats).9 living rats were randomly selected at 3hours,24hours and 72hours after the intervention from each group, blood samples and the hippocampus were taken .The expression of HMGB1 in hippocampus was measured by Western-blot, and the content of HMGB1 in serum was measured by enzyme-linked immunosorbent assay (ELISA). Results The expression of HMGB1 in hippocampus and serum at each time point of group B was significantly higher than that of group A (P\u2009<\u20090.01 ). And there was no significant difference among of 3h, 24h and 72h. The expression of HMGB1 in hippocampus and serum of group C and D was lower than that of group B at each time point, the difference was statistically significant (P\u2009<\u20090.05). In group D, the expressions of HMGB1 in hippocampus and serum of group D were lower than that of group C at 3hours and 24 hours, the difference was statistically significant (P\u2009<\u20090.05), But there was no significant difference between group C and group D at 72 hours (P\u2009>\u20090.05). Conclusion HMGB1 was significantly increased in hippocampus and serum of epileptic rats, it was suggested that HMGB1 was involved in the inflammatory process of epilepsy and HMGB1 could be used as a marker of brain injury degree after seizure. NGF and FPS-ZM1 had the effect of repairing the brain after seizure. FPS-ZM1 is superior to NGF in the protection of nervous system.