Resveratrol Supplementation Prevents Retinal Tissue Damage by Increasing Antioxidant Activity Through SIRT1 Gene Expression in Diabetic Elderly Female Rats.

Abstract

\n The present study was carried out to investigate how resveratrol administration affects retinal SIRT-1 levels and retinal tissue damage in diabetic elderly female rats.A total of 24 elder female rats were divided equally into 4 groups: 1, Control; 2, Control\u2009+\u2009Resveratrol; 3, Diabetes; 4, Diabetes\u2009+\u2009Resveratrol. Experimental diabetes was induced by a single intraperitoneal injection of Streptozotocin (STZ) in Group 3 and 4. The group 2 and 4 given intraperitoneal (ip) RSV (5 mg/kg/day) for 4 weeks in addition to the normal diet. After 4-weeks resveratrol treatment, SIRT1 gene expression and MDA ve GSH levels were determined by PCR and ELISA, respectively, in retinal tissue samples of the animals.The highest retinal MDA values were in the diabetes group (G3), the highest retinal GSH levels were in the Diabetes\u2009+\u2009Resveratrol group (G4). The retinal MDA and GSH levels of the other groups were not different from each other. The highest retinal SIRT1 expression values were in the Diabetes\u2009+\u2009resveratrol (G4) group. The retinal SIRT1 expression values of the diabetes group (G3) were lower than group 4, and higher than the groups 1 and 2. Retinal SIRT1 expression values of the groups 1 and 2 were not different from each other.Resveratrol supplementation prevented retinal tissue damage that occurs in diabetic aged female rats. This antidiabetic effect of resveratrol supplementation occurs by increasing both antioxidant activity and SIRT1 expression in diabetic aged rats.