Constraint-Based Modeling of Yeast Mitochondria Reveals the Dynamics of Protein Import and Iron-Sulfur Cluster Biogenesis

Abstract

Highlights \n• Reconstruction of mitochondrial protein import and cofactor metabolism in the model eukaryote Saccharomyces cerevisiae \n• Quantification of the proton motive force shows the significant energy cost of metabolite transport \n• Protein import activity depends on growth rate and respiratory activity \n• Quantification iron-sulfur cluster requirements show growth rate dependence