Immune-Related Molecular Profiling of Thymoma With Myasthenia Gravis

Abstract

Background: Approximately 50% of thymoma patients also show myasthenia gravis (MG), which is an autoimmune disease; however, the pathogenesis of MG-associated thymoma remains elusive. Our aim was to investigate immune-related lncRNA profiles of a set of candidate genes for better understanding of the molecular mechanism underlying the pathogenesis of thymoma with or without MG. \n \nMethods: Molecular profiles of thymoma with or without MG were downloaded from The Cancer Genome Atlas, and Pearson’s correlation analysis was performed to identify immune-related lncRNAs. T test was used to examine the differential expression and differential methylation between thymoma patients with or without MG. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to predict the function of target genes of immune-related lncRNAs. \n \nResults: Analyses of the 87 thymoma samples with complete MG information revealed that 205 mRNAs and 56 lncRNAs showed up-regulated expression in thymoma with MG patients, while 458 mRNAs and 84 lncRNAs showed down-regulated expression. The methylation level of three immune-related lncRNAs (AP000787.1, AC004943.1, WT1-AS, FOXG1-AS1) was significantly decreased in thymoma tissues, and the methylation level of these immune-related lncRNAs (WT1-AS: Cor=0.368, P<0.001; FOXG1-AS1: Cor=0.288, P<0.01; AC004943.1: Cor=-0.236, P<0.05) correlated with their expression. GO and KEGG pathway analysis revealed that targets of the immune-related lncRNA FOXG1-AS1\xa0were\xa0enriched\xa0in\xa0small GTPase binding and herpes simplex virus 1\xa0infection. Transcription coregulator activity and cell cycle were the most enriched pathways or targets of lncRNA AC004943.1.\xa0LncRNA WT1-AS\xa0targets were most enriched in actin binding and axon guidance. \n \nConclusion: Our results revealed the immune-related molecular profiling of thymoma with MG and without MG and identified key pathways involved in the underlying molecular mechanism of thymoma-related MG. These findings provide insights for further research of potential markers for thymoma-related MG. \n \nFunding Information: This study was supported by grants from the Fujian Provincial Natural Science Foundation Project [grant number 2019J01436], Fujian Provincial Health Research Talents Training Programme Medical Innovation Project [grant number 2019-CX-33] and Joint Funds for the innovation of science and Technology, Fujian province [grant number 2019Y9022]. \n \nDeclaration of Interests: The authors have no conflict of interest to disclose.