Ultrasonically Induced Covalent Grafts of Cisplatin Loaded Ginger Oil Nanoemulsion System on the Surface of OH-SWCNT’S for Anticancer Studies and Biomedical Applications - A Novel Approach in Nanotechnology

Abstract

Hydroxyl functionalized Carbon nanotubes are the low-dimensional sp2 carbon nanomaterial’s exhibiting many distinctive physical and chemical characteristics which are intriguing in a broad variety of fields including nanomedicine. Their latent applications in Nanoelectronics, biosensors, bimolecular recognition devices, and molecular transporters, as well as cancer treatment and diagnosis, are still fascinating. For the first time, Hydroxyl functionalized SWCNT(OH-SWCNT) was developed as a delivery platform for Cisplatin loaded Ginger (o/w) nanoemulsion in this work, which is known for its prominent role as targeted drug delivery systems in the field of nanomedicine application and cancer therapy. OH-functionalized SWCNTs were first modified with anionic surfactant (tween80) to facilitate the covalent connection of a Cisplatin cored GE-NE(Cisp/GE-NE) on surface charge interaction. The total Cis/Ge-ne loading on the resulting nanotubes was l mol/mg where the release was observed under both room temperature and at 37oc. the successful grafting of Cisp-Ge/Ne on OH-SWCNTs at each step was confirmed qualitatively by (Fourier-transform infrared spectroscopy and UV-visible spectroscope). Morphological studies carried by Transmission electron microscopy (TEM) have shown the hollow long tubular structure occupied with the Cisplatin cored Ginger oil nanoemulsion (Cisp/Ge-ne). Cis/Ge-ne-OH-SWCNTs were preserved after modification at different temperatures given high stability at 37o C. OH-SWCNT-Cis/Ge-Ne showed significant toxicity towards HEK(Normal Kidney cell lines) and AGS(gastric cancer cell lines) whereas no significant cytotoxicity on normal Kidney cell lines (HEK) was observed on different dilution factor equal to or below 2mg/ml. The Cis/Ge-Ne attached to hydroxyl functionalized SWCNTs is a novel and effective drug delivery method that might be employed alone or in combination with other treatments for various nanomedicine applications and also for dual-targeted therapy in cancer.