Hydrazone Analogues: Molecular Modeling, Synthesis, In- vivo Anti-Nociceptive Activity and in-vitro Antimicrobial Activity

Abstract

The inevitable consequence of the widespread use of antimicrobial agents has been the emergence of antibiotic resistant pathogens, functioning an ever- increasing used for new drugs. In an effort to develop antimicrobial agents a series of hydrazones derivative (4a-e) were synthesized from chalcones. Substituted hydrazides were reacted with chalcone in the presence of acetic acid and hydrazone derivatives were synthesized. The synthesized hydrazones were characterized on the basis physical and spectroscopic data and were evaluated for their antimicrobial activity against various bacterial and fungal strains using disc diffusion method using nutrient agar media. In addition, the antinociceptive activities of the products were evaluated. Our data showed that many derivatives have promising activities as antinocicepative agents.Furthermore the assessment of structural similarity of the target compounds with various standard drugs was done. Evaluation of the compounds revealed remarkable antibacterial, antifungal and antioxidant activity. In addition, an in silico docking study was performed in order to explain the possible interactions and the docking scores of all the compounds into the crystal structure of DNA Gyrase (Pdb code : 3U2D) using Online docking server program.