LARVICIDAL EFFECT OF ALKALOIDS EXTRACTED FROM BITTER LUPIN SEEDS AGAINST MOSQUITOES (CULEX PIPIENS), FLIES (MUSCA DOMESTICA) AND FLEAS (XENOPSYLLA CHEOPIS) UNDER LABORATORY CONDITIONS IN EGYPT

Abstract

The present work investigated the larvicidal efficacy of alkaloids extracted from Lupin seeds by\xa0water (aqueous extract) and methanol (methanol extract) against mosquitoes larvae (Culex\xa0pipiens), flies larvae (Musca domestica) and fleas larvae (Xenopsylla cheopis) under laboratorycondition in Egypt. Alkaloids were analyzed by gas chromatography – mass spectrometry (GCMS).\xa0Analysis revealed the presence of lupanine (100%), 13-OH-Lupanine (16.48%) and13α- Angeloyloxylupanine\xa0(1.3%) in methanol extract of Lupinus luteus and α- isolupanine (100%) only in\xa0aqueous extract of lupinus luteus. The lethal concentration LC50 and LC90 were obtained from the\xa0established regression log concentrate-response lines after 24 hours. Data indicated that the efficacy\xa0of methanol extract of alkaloids against Culex pipiens larvae was more effective than the effect\xa0of aqueous extract of alkaloids. The values of lethal concentration of methanol extract of alkaloids\xa0were 0.79 and 1.17 mg/ml for LC50 and LC90 respectively, while the values of lethal concentration\xa0of aqueous extract of alkaloids were 5.43 and 8.50 mg/ml for LC50 & LC90 respectively. Data also\xa0indicated that the efficacy of methanol extract of alkaloids against Musca domestica larvae was effective\xa0than the effect of aqueous extract of alkaloids. The values of lethal concentration of methanol\xa0extract of alkaloids were 6.40 and 19.18 mg/ml for LC50 &LC90 respectively, while the same\xa0values of aqueous extract of alkaloids were 11.20 and 34.00 mg/ml for LC50 and LC90 respectively.\xa0In addition, the efficacy of methanol extract of alkaloids against Xenopsylla cheopis larvae was\xa0more effective than the effect of aqueous extract of alkaloids. The lethal values concentration of\xa0alkaloids methanol extract were 10.56, 19.20 and mg/ml for LC50 &LC90 respectively, but alkaloids\xa0values of aqueous extract were 23.21 and 41.69 mg/ml for LC50 & LC90 respectively.