Journal of the Egyptian Society of Parasitology | 2019
MILÁČEK-VITOVEC STAINING METHOD VERSUS CONVENTIONAL AND MOLECULAR ONES FOR DIAGNOSIS OF CYCLOSPORA CAYETANENSIS INFECTION
Abstract
Stool samples from 150 immunocompromised patients (including 120 Gastrointestinal [GI]\xa0symptomatic and 30 GI asymptomatic) suspected to have Cyclospora cayetanensis infection\xa0were examined to evaluate the validity of Milacek-Vitovec staining method in comparison with\xa0kinyoun stain for the diagnosis of cyclosporiasis. All results were confirmed with nested PCR as\xa0a gold standard. Validation parameters included sensitivity, specificity, accuracy index, positive\xa0predictive value and negative predictive value. In addition, infection intensity was compared\xa0among all positive samples. DNAs of Cyclospora oocysts were detected among 30/120 (25%)\xa0of GI symptomatic patients versus 1.7 % of GI asymptomatic (5/30). Milacek-Vitovec stain\xa0showed more validity than kinyoun with a sensitivity reached 80% versus 73.4% (28 versus 25\xa0from 35 cases) while the specificities of showed 90.4% (104/115) versus 81.7% (94/115), respectively.\xa0Mild infection intensity was detected among 10 patients only three of them (30%)\xa0were detected by Milacek-Vitovec stain while only one (10%) was detected by Kinyoun, Moderate\xa0infection were identified among 10 patients all of them were diagnosed by Milacek-Vitovec stain and 9 (90%) was detected by Kinyoun stain. Heavy infection was detected among\xa015 patients all diagnosed with Milacek-Vitovec stain while 14 were detected with kinyoun.