pH sensitive interpenetrating network bio containers of gum ghatti for sustained release of glipizide.

Abstract

A novel natural polymer, gum ghatti (GG) was explored to develop a new polymeric system that will combine the biodegradable and biocompatible properties of GG and mechanical properties of poly vinyl alcohol (PVA) for drug delivery application. Smart pH sensitive, porous, glutaraldehyde (GA) crosslinked interpenetrating network (IPN) microspheres loaded with glipizide were developed by emulsion crosslinking method. The drug entrapment efficiency was 92.85±1.5 %. FTIR confirmed the formation of IPN structure. Microspheres have demonstrated drug release upto 7 hours depending upon the concentration of crosslinking agent. Swelling study and diffusion co-efficient (D) of water transport were performed in order to understand the phenomenon of water penetration through the microsphere. Values of D decreased with increase in the degree of crosslinking of the matrix. In vivo antidiabetic activity performed on alloxane induced diabetic rats indicated that in case of pure glipizide sudden reduction of elevated blood glucose was observed after 3 hours. In case of rats treated with glipizide loaded IPN microparticles, initial percentage reduction of blood glucose level was slow within the first 3 hours of administration, as compared to pure glipizide but after 6 hours 90% reduction was observed which confirmed sustained release nature of microspheres.