Current Proteomics | 2021
Curcumin Reverts the Protein Differential Expression in the Liver of the Diabetic Obese db/db mice
Abstract
\n\n In type 2 diabetic mouse liver, hyperglycemia, and insulin modify gene expression. Curcumin is a\npowerful antioxidant and antidiabetic agent that regulates the gene expression of different signaling pathways through\nvarious transcription factors. Therefore, we hypothesized that curcumin modifies the protein expression profile in the liver\nof diabetic db/db mice.\n\n\n\nTo determine the effects of curcumin on the liver protein profile of diabetic db/db mice.\n\n\n\n\ndb/db and wild type (WT) male mice were allocated in four groups, and they were fed for eight weeks. Three WT\nand three diabetic db/db mice received a standard diet (SD; WT and db/db groups, respectively); three WT and three\ndiabetic db/db mice received a SD supplemented with 0.75 % (w/w) curcumin (WT+C and db/db+C groups, respectively).\nLiver proteins were separated by 2D electrophoresis. Differential protein expression analysis was performed on\nImageMaster 2D Platinum software, and selected proteins were identified by MALDI-TOF-MS and subjected to enrichment\nanalysis using STRING and DAVID databases.\n\n\n\nThirty-six proteins with differential expression due to the diabetic background and curcumin treatment were found;\nthese proteins participate in the metabolism of amino acids, carbohydrates, and lipids. Interestingly, the altered expression of\nseven proteins was prevented in the liver of the diabetic mice that received curcumin.\n\n\n\nAmong all differentially expressed proteins, curcumin reverted the altered expression of seven proteins. Thus,\nalthough it was observed that curcumin did not affect the biochemical parameters, it does modify the expression of some\nliver proteins in diabetic mice.\n