Hydrophilic Interaction Liquid Chromatography (HILIC): Latest Applications in the Pharmaceutical Researches

Abstract

\n\n Significant advances have been occurred in analytical research since the 1970s\nby Liquid Chromatography (LC) as the separation method. Reverse Phase Liquid Chromatography\n(RPLC) method, using hydrophobic stationary phases and polar mobile phases, is the most commonly\nused chromatographic method. However, it is difficult to analyze some polar compounds with this\nmethod. Another separation method is the Normal Phase Liquid Chromatography (NPLC), which involves\npolar stationary phases with organic eluents. NPLC presents low-efficiency separations and\nasymmetric chromatographic peak shapes when analyzing polar compounds. Hydrophilic Interaction\nLiquid Chromatography (HILIC) is an interesting and promising alternative method for the analysis of\npolar compounds. HILIC is defined as a separation method that combines stationary phases used in the\nNPLC method and mobile phases used in the RPLC method. HILIC can be successfully applied to all\ntypes of liquid chromatographic separations such as pharmaceutical compounds, small molecules, metabolites,\ndrugs of abuse, carbohydrates, toxins, oligosaccharides, peptides, amino acids and proteins.\n\n\n\nThis paper provides a general overview of the recent application of HILIC in the pharmaceutical\nresearch in the different sample matrices such as pharmaceutical dosage form, plasma, serum,\nenvironmental samples, animal origin samples, plant origin samples, etc. Also, this review focuses on\nthe most recent and selected papers in the drug research from 2009 to the submission date in 2020,\ndealing with the analysis of different components using HILIC.\n\n\n\nThe literature survey showed that HILIC applications are increasing every\nyear in pharmaceutical research. It was found that HILIC allows simultaneous analysis of many compounds\nusing different detectors.\n