Cancer Therapy with CRISPR/Cas9: Prospects and Challenges

Abstract

Globally, Cancer is believed to be second biggest reason of mortality and one of the significant social as well as economic liabilities. Despite our advance at molecular level in comprehension of cancer, more therapeutic tools and tactics are needed to exploit this advance. The CRISPER/Cas9 genome modifying approach has lately appeared as an effective cancer therapy method due to its high accuracy and efficiency. CRISPER/Cas9 has enormous clinical potential in discovering new targets for cancer treatment and also to dismember genetic-chemical interaction thus helping us to understand the response of tumor to the treatment by drugs. Additionally, Cas9/CRISPER can also be used in cancer immunotherapeutic applications by engineering immune cells and oncolytic viruses. Perhaps the most important therapeutic application of Cas9/CRISPER is its ability to edit genes with great precision both in animal models and humans. In this review, we will debate and explore some important concerns of using CRISPER/Cas9 in remedial settings and some vital hurdles that are needed to overcome before it is used for a clinical trial for a polygenic and complex ailment like cancer. Keywords— Advance therapy; CRISPER; Cas9; Genome engineering; Cancer models. I. BENEFITS AND TECHNIQUES OF CRISPER