PORPHYROMONAS GINGIVALIS: BIOFILM FORMATION, ANTIMICROBIAL SUSCEPTIBILITY OF ISOLATES FROM CASES OF LOCALIZED AGGRESSIVE PERIODONTITIS (LAP)

Abstract

Background: No information is available on the antimicrobial sensitivity pattern and guidelines for oral antibiotic therapy for Yemeni patients for those with localized aggressive periodontitis (LAP) due to Porphyromonas gingivalis, a condition that often requires complementary antibiotic treatment. \nAim: The primary aim of the current study was to examine the antibiotic patterns and the potential relationship between P. gingivalis biofilm formation and the incidence of antibiotic resistance of clinical isolates on a group of antibiotics commonly used in oral/systemic therapy. \nSubjects and Methods: The study included 30 clinically diagnosed patients, and 30 strains of P. gingivalis were isolated from them.\xa0 Microbial sampling, isolation, and identification of bacteria were performed using culture methods appropriate to anaerobic species. Biofilm production was evaluated by the phenotypic method, that is, tissue culture methods (TCPM). Also; each isolate was tested against 12 antibiotics using the disc diffusion method. \nResults: After isolated P. gingivalis were subjected to biofilm detection by TCP method, 7 (23.3%) showed high, 6 (20%) moderate, while 17 (56.7%) showed non/weak biofilm-forming ability. P. gingivalis \xa0biofilms showed a higher resistance rate than forming non/weak biofilms e.g amoxicillin (92.3% vs 64.7%, p = 0.08), azithromycin (58.8% vs 11.7, p =0.003), metronidazole (76.9% vs 29.4%, p = 0.08), 0.01) and clindamycin (84.6% vs 47.1, p = 0.03). \nConclusion: It was found that the drug-resistant factor in P. gingivalis isolates is associated with the formation of P. gingivalis biofilm. Even though the current results show a high sensitivity result for P. gingivalis strains, some resistance has been observed. Antibiotic resistance patterns can modify over the years, make susceptibility testing essential and to promote careful choice of preliminary antibiotic treatment, the same as an adjuvant to endodontic therapy. \n\xa0\xa0\xa0\xa0\xa0\xa0\xa0\xa0\xa0\xa0\xa0\xa0\xa0\xa0\xa0\xa0\xa0\xa0 \nPeer Review History: \nReceived: 9 July 2021; Revised: 12 August; Accepted: 6 September, Available online: 15 September 2021 \nAcademic Editor:\xa0 Dr. Ali Abdullah Al-yahawi,\xa0Al-Razi university, Department of Pharmacy, Yemen, [email protected] \nUJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency.\xa0 \nReceived file:\xa0\xa0\xa0\xa0\xa0 \xa0 \xa0\xa0\xa0\xa0\xa0\xa0\xa0 Reviewer s Comments: \nAverage Peer review marks at initial stage: 6.0/10 \nAverage Peer review marks at publication stage: 7.0/10 \nReviewers: \nDr. Rawaa Souhil Al-Kayali, Aleppo University, Syria,\[email protected] \nDr. Wadhah Hassan Ali Edrees, Hajja University, Yemen, [email protected] \nRola Jadallah,\xa0Arab American University, Palestine,\[email protected] \nSimilar Articles: \nRISK FACTORS OF PERIODONTAL DISEASES AMONG YEMENI YOUNG DENTAL PATIENTS