Journal of Animal and Feed Sciences | 2021
Evaluation of Boswellia serrata enriched diet on cytokine gene\nexpression and reactive oxygen metabolites in weaning piglets
Abstract
Weaning is a stressful and complex period characterized by changes in diet, social, and environmental living conditions, which suddenly impact piglet health and lead to decreased performance and even mortality. The abrupt switch from highly digestible liquid milk to less-digestible solid feed has critical consequences on feed intake, resulting in a fasting period of 24–48 h and thus in a transiently reduced growth rate (Lallès et al., 2007). Several gastrointestinal changes are associated with the weaning transition, such as inflammation and atrophy in the small intestine, reduced villous height and recurrent diarrhoea. Furthermore, it was indicated that increased production of reactive oxygen species (ROS) is involved in the above mentioned weaning-induced gut dysfunctions, resulting in intestinal oxidative stress (Zhu et al., 2012). After the ban of antibiotics at subtherapeutic doses in livestock feeds in Europe in 2006, the search for alternative compounds with anti-inflammatory, antioxidant and immunomodulatory properties has been stimulated. In particular, interest in the use of plant-derived ABSTRACT. Weaning is a stressful period that impacts piglet health leading to decreased performance and sometimes mortality. In recent years, public and scientific interest in dietary plant extracts tremendously increased because of their biological benefits. We hypothesized that the extract of the Indian frankincense Boswellia serrata, especially known for its anti-inflammatory properties, could affect piglets in the postweaning period when inflammation plays a crucial role. Eighty weaned piglets (40 gilts and 40 barrows, initial average body weight 10.0 ± 0.43 kg) were randomly assigned to one of two dietary treatments: control (CON) or CON diet supplemented with 1 kg/t of B. serrata (BOSW). The trial lasted 28 days. Blood samples were collected at the beginning and the end of the trial. Skin lesions and thermal imaging were evaluated weekly. Growth performance, wellbeing parameters and gene expression levels of cytokines were not affected (P > 0.05), except for a trend in lower gene expression of interleukin(IL)-10 in BOSW group (P = 0.07). Time effect (P < 0.01) was observed for gene expression of IL-4, IL-6, IL-8 and tumour necrosis factor (TNF)-α, and sex effect (P < 0.05) was noted for gene expression of IL-1β, IL-6 and TNF-α, showing higher values for male than female piglets. Time changes in reactive oxygen metabolites (ROMs) blood concentration (ΔROMs) tended to be slower in BOSW group than in CON group (P = 0.056). These preliminary results indicate the need to investigate further doses and length of supplementation of B. serrata to verify the potential effects reported in other livestock species. Received: 20 November 2020 Revised: 22 March 2021 Accepted: 8 April 2021