Archive | 2021
A label-free multimodal nonlinear microscope for biological applications
Abstract
INTRODUCTION: We developed a home-made, innovative and label-free transmission inverted multimodal nonlinear microscope with off-the-shelf components able to perform twophoton excitation fluorescence (TPEF), second harmonic generation (SHG), coherent antiStokes Raman scattering (CARS) and stimulated Raman scattering (SRS) microscopy [1]. This set-up is highly versatile, can be easily reconfigured and allows the observation of vital, complex and thick biological samples without invasive laser sources and staining. In this work, we performed nonlinear imaging of different biological samples, from single cells to highly heterogeneous tissues to validate our acquisition system.