Anticoagulant and cytotoxic assessment of L-amino acid oxidase from Indian cobra (Naja naja) venom

Abstract

Introduction: Snake venom L-amino acid oxidase (SV-LAAO) has promising therapeutic prospects because of its effects on various biological functions. Purified Naja naja venom enzyme L-amino acid oxidase (NnLAAO) was studied to examine its anticoagulant and cytotoxic properties. Methods: Activated partial prothrombin time (APPT) assay and prothrombin time (PT) both of these assays are used to access the anti-coagulant property of NnLAAO on human blood sample. Sulforhodamine B (SRB) protocol is performed on MDA MB-231and HCT 116 cell, which are treated with different doses (0.4µg-100µg) of NnLAAO for 48h incubation time, respectively, and the percentage of inhibition was compared with oxaloplatin as positive control. Results: NnLAAO significantly prolonged the APPT and showed a negligible effect on the PT. NnLAAO inhibited cytotoxicity in a dose-dependent manner in both breast cancer MDA MB-231 and colon adenocarcinoma (HCT-116) cell lines with 60% and 78% of inhibition respectively. Conclusion: The NnLAAO enzyme of Indian cobra venom is a promising anticoagulant as well an anticancer agent for various cell lines because of its efficiency and specificity, as it will offer better treatment and response with fewer side effects.