Effects of ibogaine per os treatment on redox homeostasis in rat kidney

Abstract

Paper description: Ibogaine in vivo induces liver glycogen depletion, provokes mild oxidative stress and activates antioxidant enzymes. We examined the in vivo effects of a single per os dose of ibogaine (1 or 20 mg/kg) on the morphology and antioxidative system of rat kidney. Ibogaine applied at a dose of 1 mg/kg elevated superoxide dismutase 1 activity, which was followed by a decrease in glutathione reductase activity, which was also decreased after application of 20 mg/kg, suggesting disturbed redox homeostasis. Ibogaine treatment with both doses provoked moderate changes in kidney morphology. These findings indicate a precautionary approach with ibogaine use regarding kidneys. Abstract: Our previous results showed that a single oral dose (1 or 20 mg/kg body weight) of the anti-addiction agent ibogaine induced in rats 6 and 24 h after administration glycogenolytic activity in hepatocytes, followed by a mild oxidative stress. In this work, we examined the in vivo effect of the same doses of ibogaine on rat kidney morphology, antioxidant enzyme (superoxide dismutases (SOD 1 and 2), catalase, glutathione peroxidase, glutathione reductase (GR) and glutathione-S-transferase) activities, and oxidative stress (TBARS) and redox (-SH groups) parameters. The dose of 1 mg/kg ibogaine induced an elevation in SOD1 activity and decreased GR activity after 6 and 24 h. GR activity was decreased at 6 and 24 h after 20 mg/kg ibogaine administration, suggesting changed redox homeostasis. After 24 h, we observed an increase in moderate morphological changes, without changes in urinalyses, indicating that kidney function was not measurably affected. Nevertheless, kidney-function monitoring during and following ibogaine use in human subjects is advisable. https://doi.org/10.2298/ABS190208006V Received: February 8, 2019; Revised: February 17, 2019; Accepted: February 19, 2019; Published online: February 21, 2019 How to cite this article: Vidonja Uzelac T, Tatalovic N, Mijovic M, Nikolic-Kokic A, Orescanin-Dusic Z, Bresjanac M, Blagojevic D. Effects of ibogaine per os treatment on redox homeostasis in rat kidney. Arch Biol Sci. 2019;71(2):245-52.