Diabetes Care | 2019
Response to Comment on Mathew et al. Therapeutic Lifestyle Changes Improve HDL Function by Inhibiting Myeloperoxidase-Mediated Oxidation in Patients With Metabolic Syndrome. Diabetes Care 2018;41:2431–2437
Abstract
We thank Holzer and Marsche (1) for their interest in our work on the impact of therapeutic lifestyle changes (TLC) on HDL function in patients with metabolic syndrome (2). They suggest that a modified isolation technique for HDL2 and HDL3 proposed by their group removes proteins such as albumin (most abundant plasma protein) and apolipoprotein (apo)B (the major constituent of LDL), thereby enhancing HDL purity. It is well known that HDL protein composition is highly heterogeneous, and different HDL isolation techniques—such as sequential ultracentrifugation (KBr or D2O/sucrose), size exclusion chromatography, immune affinity capture, ion exchange chromatography, or isoelectric focusing—yield variable combinations of ∼95 proteins, as reviewed (3) and as updated periodically on the website http://homepages.uc.edu/∼davidswm/HDLproteome.html. These proteins …