Efek Oktil Galat Sintetik terhadap Interleukin-1 Beta dan Interleukin-1 Receptor Antagonist pada Tikus Model Endometriosis

Abstract

Endometriosis merupakan penyakit imun-inflamasi kronik yang ditandai dengan pertumbuhan jaringan endometrial di luar rongga uterus. Pada endometriosis terjadi peningkatan kadar IL-1β sedangkan kadar IL-1Ra menurun atau tidak terdeteksi; ketidakseimbangan tersebut menginduksi pertumbuhan dan perkembangan lesi endometriosis. Penelitian ini bertujuan untuk menganalisis efek oktil galat (OG) terhadap kadar IL-1β dan IL-1Ra dari supernatan homogenat jaringan tikus model endometriosis. Penelitian eksperimental dilakukan di Animal Research Facilities Indonesia Medical Education and Research Institute, Fakultas Kedokteran Universitas Indonesia pada bulan Juni 2018-Mei 2019. Sebanyak 30 tikus wistar betina dibagi menjadi tiga kelompok. Kelompok I dilakukan operasi sham, sedangkan kelompok II dan III diautotransplantasi endometriosis. Kelompok II diinduksi carboxymethyl cellulose sodium Na-CMC dan kelompok III diberikan suspensi 20 mg OG dalam Na-CMC per oral selama satu bulan. Seluruh hewan coba dieutanasia, jaringan endometrium dari kelompok I serta endometriosis dari kelompok II dan III diambil untuk dihomogenasi. Kadar IL-1β dideteksi dengan metode Luminex, sedangkan IL-1Ra dengan ELISA. Rerata kadar IL-1Ra pada kelompok III (243,601 ± 92,505 ng/mgP) lebih tinggi dibandingkan kelompok II (178,058 ± 87,545 ng/mgP). Uji beda proporsi kadar IL-1Ra kategori tinggi pada kelompok III (33,3%) secara signifikan lebih tinggi dibandingkan kelompok II (12,5%). Induksi OG pada tikus model endometriosis meningkatkan kadar IL-1Ra, namun tidak menekan kadar IL-1β. The Effect of Octyl Gallate Synthetic to Interleukin-1 Beta and Interleukin-1 Receptor Antagonist in Rat Endometriosis Model Endometriosis is a chronic immune-inflammatory disease characterized by the growth of endometrial tissue outside the uterine cavity. There is an increase of IL-1β levels while IL-1Ra levels are decreased or not detected in endometriosis. This imbalance induces the growth and development of endometriotic lesions. This study aims to analyze the effect of octyl gallate (OG) on the levels of IL-1β and IL-1Ra from homogenate supernatant of the mouse tissue endometriosis model. Experimental research was carried out at the Animal Research Facilities Indonesia Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia in June 2018-May 2019. A total of 30 female wistar rats were divided into three groups. Group I underwent sham surgery, while groups II and III had autotransplantation for endometriosis. Group II was induced by carboxymethyl cellulose sodium Na-CMC and group III was given a suspension of 20 mg OG in Na-CMC orally for one month. All experimental animals were euthanized, endometrial tissue from the group I and endometriosis from groups II and III were taken to be homogenized. IL-1β levels were detected by the Luminex method, while IL-1Ra levels were detected by ELISA. The mean levels of IL-1Ra in group III (243.601 ± 92.505 ng / mgP) were higher than in group II (178.058 ± 87.545 ng / mgP). A different test for the proportion of high category IL-1Ra levels in group III (33.3%) was significantly higher than group II (12.5%). OG induction in endometriosis model mice increased IL-1Ra levels, but did not suppress IL-1β levels.