Evaluation of glucose absorption rates following intracoelomic or subcutaneous administration in experimentally dehydrated inland bearded dragons (Pogona vitticeps).

Abstract

OBJECTIVE\nTo evaluate glucose absorption rates as an indirect measure of fluid absorption after SC or intracoelomic (ICe) administration of 2.5% dextrose solution to experimentally dehydrated inland bearded dragons (Pogona vitticeps).\n\n\nANIMALS\n9 adult bearded dragons.\n\n\nPROCEDURES\nIn a randomized, blinded, placebo-controlled, crossover design study, bearded dragons were experimentally dehydrated with a previously described protocol and then received 2.5% dextrose solution (a 1:1 mixture of 5% dextrose in isotonic multiple electrolytes solution; 20 mL/kg), SC or ICe, or a control treatment (the same electrolytes solution without added glucose; 20 mL/kg). Blood glucose (BG) concentrations were measured at predetermined times up to 24 hours after fluid administration. There was a ≥ 14-day washout period between treatments. Mean changes in BG concentration from baseline were compared among treatments.\n\n\nRESULTS\nAdministration of 2.5% dextrose solution by either route increased BG concentration with a significantly greater change in values within 5 minutes, compared with control treatment results. The mean change in BG concentration after ICe administration was significantly greater than that after SC administration 15 and 30 minutes after injection, with mean differences of -50 and -36 mg/dL, respectively, for the SC treatment. Within 1 hour after fluid administration, there was no significant difference in BG values between the 2 dextrose administration routes.\n\n\nCONCLUSIONS AND CLINICAL RELEVANCE\nFindings supported that fluid therapy by SC administration, which carries a lower risk of iatrogenic complications, can provide results similar to those achieved with ICe administration.