Estimating leaf water content at the leaf scale in soybean inoculated with arbuscular mycorrhizal fungi from in situ spectral measurements

Abstract

Leaf water content (LWC) of crops is a suitable parameter for evaluation of plant water status and arbuscular mycorrhizal effect on the host plant under drought stress. Remote sensing technology provides an effective avenue to estimate LWC in crops. However, few LWC retrieval models have been developed specifically for the arbuscular mycorrhizal inoculated crops. In this study, soybean with inoculation and non-inoculation treatments were planted under the severe drought, moderate drought and normal irrigation levels. The LWC changes under different treatments at the 30, 45 and 64 day after the inoculation were investigated, and the spectral response characteristics of inoculated and non-inoculated soybean leaves under the three drought stresses were analyzed. Five types of spectral variables/indices including: raw spectral reflectance (R), continuum-removed spectral reflectance (RC), difference vegetation index (DVI), normalized difference vegetation index (NDVI) and ratio vegetation index (RVI) were applied to determine the best estimator of LWC. The results indicate that LWC decreased as the aggravating of drought stress levels. However, LWC in inoculated leaves was higher than that in the counterparts under the same drought stress level, and the values of raw reflectance measured at inoculated leaves were lower than the non-inoculated leaves, especially around 1900 nm and 1410 nm. These water spectral features were more evident in the corresponding continuum-removed spectral reflectance. The newly proposed DVIC(2280, 1900) index, derived from the continuum-removed spectral reflectance at 2280 nm and the raw spectral reflectance at 1900 nm in DVI type of index, was the most robust for soybean LWC assessment, with R value of 0.72 (p < 0.01) and root mean square error (RMSE) and mean absolute error (MAE) of 2.12% and 1.75%, respectively. This study provides a means to monitor the mycorrhizal effect on drought-induced crops indirectly and non-destructively.