Phenotypic and Genotypic Characterizations of Non-Biofilm Producer Porphyromonas gingivalis Associated with Periodontitis in Al-Najaf, Al-Ashraf/Iraq

Abstract

This study aims to investigate the correlation between some virulence factors and biofilm production among\nPorphyromonas gingivalis associated with periodontitis. One hundred and two sub gingival swabs have been collected\nfrom patients suffering from acute and chronic periodontitis admitted to Specialty Dental center in the Holy city of Najaf\nand specialized clinics in the Faculty of Dentistry of the University of Kufa from both sexes during the period from\nSeptember 2017 to January 2018. A total of 34 isolates (28.43%) were identified as P. gingivalis by amplification of 16S\nrRNA using PCR technique. luxS, lux pro and lux down genes were investigated and the results of PCR showed that none\nof the strains were possess luxS, 3 strains of P. gingivalis were possess lux pro and 2 strains of P. gingivalis were possess\nlux dwn. Only 10 strains of P. gingivalis were selected for detection of some virulence factors. The results showed that\nall strains were unable to produce biofilm with variant abilities of bacterial strains for autoaggregation have been\nobserved (decrease in O.D of bacterial growth after 1 hr. of incubation), and one strain gave +ve results for\nhemagglutination.