Comparative study Antibacterial Activity of some Medicinal Plants Extracts ( leaves and Peel ) against some Multi-drug Resistant Bacteria from Clinical Isolates

Abstract

The study was conducted of tested the effect of three aqueous and alcoholic extract of medicinal plants from (Ziziphus spina-christi , Punica granatum L. and Eucalyptus camaldulensis ) 100 mg/ ml concentration and the sensitivity of to nine Antibiotics (ciprofloxacin, Azithromycin, Augmentin, Amoxicillin+Clavulanic Acid, Ceftazidime, Vancomycin,Tobramycin (10 μg), Tobramycin(5μg), Ampicillin) against 20 antibiotic resistant pathogenic bacteria isolate by using Mueller-Hinton Agar (MHA) well diffusion method. Antibacterial activity of alcoholic (ethanoll) extraction represented by inhibition zones diameters of three medicinal plants showed strong activity comparison of aqueous extracts , Especially in the pomegranate extract as the proportion of sensitive bacteria 40% and 25% in Eucalyptus camaldolehsis leaves And less inhibitory in the alcoholic extract of Ziziphus spina-christi as estimated (5%). The ethanolic extracts of Pomegranate Peel have shown an interesting activity against Yersinia enterocolitica , Salmonella spp ,and Brucella abortus with inhibition zones diameters of 30.0, 27.0 and 25.0 mm, respectively. Ciprofloxacin, was the most effective antibiotics against almost all the studied pathogenic bacteria and less resistance to other types. It is worth mentioning the existence of results showing the efficacy of plant extracts in inhibiting bacteria resistant to all and many types of antibiotics used in this study such Staphylococcus aureus HM , Shigella spp ,Enterobacter spp, Salmonella spp T,Salmonella spp, Yersinia enterocolitica ,Listeria monocytogenus.