MiR-30a-3p promotes ovariectomy-induced osteoporosis in rats via targeting SFRP1.

Abstract

OBJECTIVE To explore the effect of miR-30a-3p on osteoporosis in rats after ovariectomy. MATERIALS AND METHODS The ovariectomized (OVX) rat model was established to mimic postmenopausal osteoporosis. The primary bone marrow mesenchymal stem cells (BMMSCs) isolated from female rats were transfected with mimic or inhibitor. Real Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and Western blotting were used to examine the expressions of miR-30a-3p and osteoporosis-related proteins. The bone mineral density (BMD) was detected via micro-Computed Tomography (CT) and the bone histomorphometry was performed. Luciferase assay was also performed to confirm whether SFRP1 is a target of miR-30a-3p. RESULTS According to micro CT, BMD significantly declined in OVX group. MiR-30a-3p and SFRP1 were negatively correlated after ovariectomy. SFRP1 was acknowledged as a target of miR-30a-3p. Besides, the miR-30a-3p inhibitor promoted osteogenic differentiation in vitro and bone formation in vivo. CONCLUSIONS MiR-30a-3p inhibitor promotes bone formation through decreasing SFRP1 expression and miR-30a-3p may be a potential novel molecular target in the treatment of osteoporosis.