Enhancing The Photocatalytic Conversion Of Pt(IV) Substrates By Flavoprotein Engineering

Abstract

Our recent work demonstrates that certain\nflavoproteins can catalyze the redox activation of Pt(IV) prodrug complexes\nunder light irradiation. Herein, we used site directed mutagenesis on the mini Singlet Oxygen Generator (mSOG) to modulate the\nphotocatalytic activity of this flavoprotein towards two model Pt(IV)\nsubstrates. Among the prepared mutants, Q103V mSOG displayed enhanced catalytic\nefficiency as a result of its longer triplet excited state lifetime. This study\nshows, for the first time, that protein engineering can improve the catalytic\ncapacity of a protein towards metal-containing substrate.