Prevalence of Intestinal Parasites in Children Living in Various Living Conditions in Slovakia

Abstract

The purpose of the present study was to identify\xa0the prevalence of intestinal parasites among the children (aged ≤ 18 years) living in various living conditions in the Eastern Slovakia. Their faecal samples were examined applying the\xa0flotation concentration technique to verify the presence of helminth eggs and cysts of protozoan parasites. The evidence of\xa0Enterobius vermicularis\xa0pinworm was obtained using the perianal tape test.\xa0The diagnostics of the\xa0Cryptosporidium\xa0spp. oocysts was carried out applying the Kinyoun staining technique. The examined faecal samples were collected from 565 children living in Medzev (340 Roma children from the segregated Roma settlement; 123 children from the majority population; 33 children from the orphanage; 35 children from the elementary school; and 34 children from the kindergarten). They were divided into 5 age subgroups. In children from the segregated Roma settlement, the eggs of six helminth species:\xa0Ascaris lumbricoides,\xa0Trichuris trichiura,\xa0Ancylostomatidae\xa0family,\xa0Hymenolepis nana,\xa0Taenia\xa0spp.,\xa0Enterobius vermicularis\xa0and two protozoan species:\xa0Giardia duodenalis\xa0and\xa0Cryptosporidium\xa0spp. were detected. The highest number of positive samples was observed in the group of children aged 6-9 years. In the majority group of children, the eggs of\xa0A.\xa0lumbricoides,\xa0Taenia\xa0spp.,\xa0E. vermicularis\xa0and the cysts of\xa0G.\xa0duodenalis\xa0were detected.\xa0The species detected in children from the orphanage included\xa0A.\xa0lumbricoides,\xa0H.\xa0nana, E.\xa0vermicularis\xa0and the cysts of\xa0G.\xa0duodenalis. Children attending the 3rd\xa0year of the elementary school and the kindergarten presented with the eggs of\xa0A.\xa0lumbricoides\xa0and\xa0E. vermicularis\xa0and\xa0the cysts of\xa0G.\xa0duodenalis. The examination of soil was performed applying the Kazacos’ technique and the samples were collected from the area surrounding the houses and drinking water sources in the segregated settlement. It revealed high occurrence of parasites in infective stages. The results presented herein indicate that the environmental factors contribute to the circulation of parasites among children and affect their health.