Characterization and antihypertensive activity of ginger extract-eel bone oil SNEDDS (selfnanoemulsifying drug delivery system)

Abstract

This research aimed to determine the characterization of Self-Nanoemulsifying Drug Delivery System (SNEDDS) of Zingiber officinale extract with eel (Anguilla spp.) bone oil and activity on systolic and diastolic blood pressure of Wistar rats with dexamethasone-induced hypertension. Hypertension was induced by giving rats subcutaneous dexamethasone injections (30 μg/kg/day) for 14 days. In prevention group, rats received oral 200 mg/kg BW of crude ginger extract (GE), 200 and 300 mg/kg BW SNEDDS ginger extract (200 SGE and 300 SGE), and 40 mg/kg BW captopril from 4 days before and during treatment period (days 1-18). In curative treatment group, SNEDDS ginger extract was administered orally from days 8-14. The results, using a UV spectrophotometer showed the transmittance of SNEDDS was 92.16%; the optimal emulsification time was 10.01 seconds in artificial gastric fluid (AGF), 10.15 seconds in distilled water, and 9.44 seconds in double-distilled water, with a measured particle size of 13.10 nm, polydisperse index 0.134 and zeta potential of -14.04 mV. The antihypertensive activity analyzed by two-way ANOVA (p<0.05) showed that 200 SGE and 300 SGE have effectiveness for preventing symptoms of hypertension. In curative group showed significant different systolic blood pressure between SNEDDS ginger extract and crude ginger extract. This indicated that the tested SNEDDS ginger extract was effective for treating symptoms of hypertension.