Ketamine Anesthesia on Neurocyte in Fetal Rat via cAMP and NO/cGMP Pathway

Abstract

Received: Revised: Accepted: Published online: May 13, 2018 October 08, 2018 October 14, 2018 December 21, 2018 Ketamine anesthesia during infancy has an impact on development of nervous system. This study is to demonstrate effects of ketamine on neuronal cells via cAMP and NO-cGMP pathway. The cortex neurocyte of Wistar fetal rat were divided into four groups. Every group was given different doses, low dose group was 0.7 μg/mL, anesthesia groups were 1 μg/mL, 3 μg/mL, 5 μg/mL at 0, 5, 10, 15, 20, 25, 30, 45, 60, 90 and 120 min, respectively. The content of cGMP and cAMP were detected by ELISA, and the activity of NOS and content of NO were detected by spectrophotometric method. After different concentrations of ketamine were applied to isolated neurons, cAMP and cGMP levels were lowered first and then increased, the content reached the bottom at 25 min and then increased. In the 30 min, the content of NO and NOS decreased to the lowest value and then went up, respectively. In the 120 min, their values were both lower than 0 min. Ketamine probably expressed its anesthesia effect with the increasement of cAMP and inhibition of NO-cGMP pathway. ©2018 PVJ. All rights reserved