La sobre-expresión de PtCSP4 del álamo promueve un mayor potencial para la fitorremediación de PCB

Abstract

Phytoremediation is a widely accepted biotechnology that reduces, absorbs and degrades toxic pollutants. Polychlorinated biphenyls (PCB) are persist organic pollutants that cause harmful effects to the environment. It has been acknowledged that poplar (Populus trichocarpa) is fast growing, it has high transpiration and a complete genome sequence. Thus, it is considered a perfect model in phytoremediation. Genetically modified plants can become more efficient remediation sources of soil and water contaminated sites with polychlorinated biphenyls (PCB). Yb-1 is a multifunctional protein involved in the regulation of transcription, translation, mARN splicing and ADN repair. Yb1 transcription factor, is known in humans and mammals but very little in plants. Yb-1 in plants is best known as CSP (cold shock proteins). In this work, PtCSP4 (Potri.004G172600) gene was isolated and amplified from poplar. The gateway recombination system was used to clone PtCSP4 in a binary vector with a constitutive promoter. PtCSP4 was introduced in Arabidopsis thaliana and its tolerance was tested in presence of PCB (Aroclor 1221). Eight transgenic lines of in vitro cultured Arabidopsis were exposed to PCBs. All results suggest that PtCSP4 is a good candidate for phytoremediation of PCB.