Archive | 2021
Classical Enterotoxin Genes of Staphylococcus aureus Isolated from the Raw Milk of Cows and Goats in Yogyakarta Indonesia
Abstract
Milk is a highly nutritious food containing several essential nutrients. Consequently, it can be contaminated by pathogenic bacteria, including Staphylococcus aureus. This study aimed to analyze the genes encoding nine classical enterotoxins of S. aureus isolated from cow and goat raw milk. A total of 75 S. aureus isolates were obtained from milk samples of dairy cows (46 isolates) and Peranakan Etawa (PE) goats (29 isolates) from different farms in Yogyakarta Indonesia. Based on the cultural and biochemical properties and the 23S rRNA occurrence, all isolates could be identified as S. aureus. The seh gene was the most frequently observed in 21 isolates (45.65%) of cows, followed by the sea gene (in 8 isolates, 17.39%), the seg and the sei genes (each in 4 isolates, 8.69%). The sec is the only staphylococcal enterotoxin gene observed in S. aureus isolated from goat milk with the prevalence of 6.89%. The genes encoding four out of nine classical enterotoxins (seh, sea, seg, and sei) are commonly isolated from S. aureus in cow milk, but only sec gene can be found in the isolates from goat milk.