BIOLOGICAL PARAMETERS AND ENZYME ACTIVITIES IN MUSCA DOMESTICA UNDER SELECTION WITH FIPRONIL

Abstract

Insect resistance to insecticides is one of the main issues of veterinary, medicine, and horticulture around the world. Knowledge of insecticidal resistance mechanisms is crucial for the development of insect control programs. The aim of the present study was to assess some biological parameters and enzyme activities in the house fly Musca domestica L. under selection with fipronil. The selection of M. domestica with fipronil was conducted by non-choice feeding when adults in each generation were fed with sugar that was pre-treated with insecticide solution. In even-numbered year generation, we evaluated the duration of individual development stages, the weight of individuals, fertility, and activity of the main detoxification enzymes (monooxygenases, esterases, and glutathione-S-transferases) in larvae and adults. The assessment of insect susceptibility to fipronil showed that larvae in the tenth generation of the fipronil-selected strain were more susceptible to fipronil than the individuals in the laboratory strain, and adults did not differ from the control as per this indicator. In the tenth generation of the fipronil-selected strain, we found that the duration of the development period from the egg stage to the emergence of adults lasted longer (by 18%) compared to the laboratory line. We noted that the activity of monooxygenases and glutathione-S-transferase in larvae and adults varied in certain generations of the fipronil-selected strain.