In vitro anti-inflammatory and anti-proliferative potential of roots extract of Euphorbia hirta Linn

Abstract

Aim: The main aim of this current study was to characterize the anti-inflammatory and anti-proliferative activities of alcoholic extract of the roots of euphorbia hirta. Genus Euphorbia contains many therapeutic plants which are widely studied. This study is designed to explore the effects of alcoholic roots extract of euphorbia hirta, on selected cell lines of Human Breast cancer (MCF - 7). Methods: The Human Breast Cancer cell lines i.e. MCF – 7 cell lines were treated with alcoholic roots extract of euphorbia hirta at different concentrations. Anti-proliferative effect of the alcoholic extract was evaluated by assessing the cell viability. MTT assay was used to determine the cell viability at the wavelength of 570 nm. Antiinflammatory potential of extract was evaluated by calculating the percentage inhibition of protein denaturation. Results: The results showed the significant anti-inflammatory potential as it effectively inhibits the protein denaturation. The alcoholic roots extract of euphorbia hirta also showed moderate cytotoxic effect against MCF - 7 cell-lines (P ≤0.05). Conclusions: The results showed that this extract had significant anti-inflammatory potential and moderate anti-proliferative effect, as extract was able to inhibit the proliferation of tumor cells. Hence, it seems to be a virtuous contender to protect against growth of tumor cells.