Drug Resistance and Biofilm Production among Pseudomonas aeruginosa Clinical Isolates in a Tertiary Care Hospital of Nepal

Abstract

Background: Clinical isolates of Pseudomonas aeruginosa often exhibit multidrug resistance due to their inherent ability to form biofilms. Drug resistance in Pseudomonas aeruginosa is a major clinical problem, especially in the management of patients with nosocomial infections and those admitted to ICUs with indwelling medical devices. To evaluate the biofilm forming abilities of the clinical isolates of Pseudomonas aeruginosa and to correlate biofilm formation with antibiotic resistance. *Corresponding to: Rajani Shrestha, Department of Microbiology, Manipal College of Medical Sciences, Pokhara, Nepal, Tel: 00 977 9856039849; E-mail: [email protected] Received: July 29, 2020; Accepted: August 11, 2020; Published: September 25, 2020 Citation: Shrestha R, Nayak N, Bhatta DR, Hamal D, Subramanya SH, Gokhale S (2020) Drug Resistance and Biofilm Production among Pseudomonas aeruginosa Clinical Isolates in a Tertiary Care Hospital of Nepal. J Drug Metab Toxicol. 11:250. doi: 10.35248/21577609.20.11.250 Copyright: 2020 © Shrestha R, et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. METHODS A total of 90 consecutive isolates of P. aeruginosa obtained from various specimens collected from patients visiting the Manipal Teaching Hospital, Pokhara, Nepal between January 2018 October 2018 were studied. Isolates were identified by standard microbiological methods. Antibiotic susceptibility testing was performed by Kirby-Bauer disc diffusion method. All the isolates were tested for their biofilm forming abilities by employing the tissue culture plate assay.