Biomics | 2021
Obtaining the rapA1 genome-transformed tomato plants
Abstract
Bacterial agglutinin RapA1 is involved in the attachment of rhizobia to the roots of macrosymbiont plants. Obtaining transgenic crops that produce this protein directly on the root surface is important for studying the symbiosis of these plants with rhizobia. Tomatoes (Lycopersicon lycopersicum L.) cultivar Gruntovy Gribovskiy 1180 were transformed with the gene rapA1 using the Agrobacterium tumefaciens AGL0 strain carrying the vector pCambia1301LPSLRapA1. The efficiency of the developed transformation method was about 5%.