DETERMINATION OF MELATONIN IN BATS GUANO SEDIMENTS BY HPLC COUPLED WITH FLUORESCENCE DETECTION: MELATONIN AS AN ADJUVANT TREATMENT OF COVID-19

Abstract

Research related to geological materials that are useful for health in Indonesia (bio-geo-prospective) for health is still being developed. This article opens a new perspective on the possibility of using melatonin in bats guano rock in attenuating COVID-19 based on the prospect of its occurrence. The current outbreak of the COVID-19 virus is a pandemic that has infected hundreds of thousands of patients worldwide which certainly requires good medication. To determine the presence of bioactive chemicals in sediment rocks both qualitatively (types) and quantitatively (levels) can be done by analyzing the active compounds (compounds (chemical compounds that have medicinal properties). While obtaining these active compounds can be done by isolating the active compounds. The isolated pure compound can be further developed into modern medicine. A sensitive and validated method for the melatonin determination by developed High-performance liquid chromatography (HPLC) coupled with fluorescence detection with an extraction procedure of direct sample extraction (DSE) in measure melatonin levels in bats guano rock. The test validation shows that the use of this method is very successful as indicated by the high correlation coefficient value of 0.9997, the RSDs (n = 3) of 4.35% and the LOD values is 0.0236 (ng / g FW) and LOQ 0.1283 (ng / g FW). The average melatonin content in guano stones was 24+ 0.18 ng / g FW.