Green synthesis and antibiofilm potential of Silver Nanoparticles loaded with Narcissus tazetta L. Extract

Abstract

Bacterial infections are the leading cause of mortality, due to the paucity of effective drugs. This study aimed to synthesize AgNPs loaded with fresh leaves & bulbs extracts of Narcissus tazetta and evaluate antimicrobial and antibiofilm potential against selected bacterial and fungal isolates using standard microbiological protocols. Different techniques like FTIR spectroscopy, GC-MS, electron microscopy and HPLC were used to characterize the AgNPs. The AgNPs synthesized from crude extract of leaves and bulb were checked and were found effective against all the bacterial isolates except E. coli however, strong biofilm inhibition potential was observed in case of E.coli. The synthesized AgNPs showed dose dependent zone of inhibition higher for methanol and lower for n-Hexane against both Gram +\u200ave and Gram -ve bacterial strains, exhibiting the anti-microbial potential. Biofilm inhibition was recorded at sub-MIC values against Gram +\u200ave and Gram -ve bacterial strains. Antifungal activity of AgNPs was observed only at a higher concentration against Aspergillus niger, Fusarium oxysporum and Alternaria alternata while Aspergillus terreus was resistant even at higher concentrations. In conclusion, we can say that the plant possesses pronounced antimicrobial potential with a wide range of the bioactive compounds, which can be explored for pharmaceutical purposes.