Immunobiotechnological methods for the determination of antibiotics in food

Abstract

In veterinary practice, a large list of antibiotics is used as therapeutic and/or prophylactic agents.Some of them are used as stimulators of the growth and productivity of animals.However, non-observance of the rules for the use of antibiotics and / or timing of slaughter or obtaining milk leads to the intake of a residual amount of antibiotics into the human body with food and can cause various pathologies. The article provides an overview of research papers published in peer-reviewed journals from 2015 to the present on the development and improvement of methods for testing milk and meat for antibiotics. Based on the literature review and the results of his own study, the author of the article notes the advantages of enzyme-linked immunosorbent assay (ELISA) options over instrumental analytical methods, such as liquid chromatography with tandem mass spectrometry and high-performance liquid chromatography. However, due to the complexity of the analysis procedure, ELISA kits are not used in food safety laboratories in the Republic of Kazakhstan and other CIS countries. The article describes the state and prospects for the development of simple rapid tests to determine the maximum residue limit (MRL) for antibiotics in livestock products, based on the use of Lateral Flow Assay (LFA). Such tests, not inferior in their sensitivity to ELISA and surpassing it in cost, could be used not only in laboratory conditions, but also directly by food consumers, which will contribute to reliable protection of public health and the development of animal husbandry.