Study on Paeoniflorin Promoting the Recovery of Motor Function of Rat Spinal Cord Injury by Mediating MAPK/ERK and Akt/mTOR Signal Pathway

Abstract

Spinal cord injury (SCI) is a devastating disease that can cause severe motor, sensory, and autonomic dysfunction. There is currently no effective treatment. Paeonia lactiflora is a traditional Chinese herbal medicine, which has antispasmodic, analgesic, and blood circulation effects. Paeoniflorin (PEF) is a medicinal plant isolated from Paeoniae Radio, and it is widely used in East Asia. A large number of studies have shown that PEF has a powerful neuroprotective effect. However, the potential mechanism of PEF on SCI needs further study. This project uses Basso Beattie Bresnahan (BBB) motor function score and open field test to evaluate neurological function, and uses immunofluorescence method to detect brain-derived neurotrophic factor (BNDF) and neurotrophin-3 (NT). -3) Protein expression, Western blot is used to detect protein expression level, and RT-PCR is used to detect mRNA expression level. Thus, a rat spinal cord injury model was established to observe the effects of AT/mTOR and MAPK/ERK signal pathways activated by PEF on nerve regeneration and functional recovery in rats with spinal cord injury.