GC-MS Analysis of Bioactive Compounds in Ethanol and Ethyl Acetate Fraction of Grapefruit (Citrus maxima L.) Rind

Abstract

The Citrus (Linn) family of Rutaceae, aromatic green shrubs and small tree plants has an important role in India s medicine. Scientifically this plant is known as Aurantium maximum Burm. Ex Rumph, Citrus aurantium L. Var grandis L., Citrus decumana L, Citrus grandis Osbeck & Citrus pamplemos. Citrus grandis Osbeck is a plant spread in India, China, Indonesia, US, Thailand, and others. The orange tree is about 16 to 50 feet tall. Oranges are native to Malayu and East India. Widespread in China, Japan, Philippines, Indonesia, US, and Thailand1,2. Citrus maxima L. or grapefruit is a plant commonly known as Papanus, spread throughout India. The bark and roots of C. maxima contain β-sitosterol, an acridone alkaloid. Essential oils from the leaves and fruits of C. maxima, which are still raw, contain limonin, nerolol, neryl acetate, and geraniol3. This plant contains vitamin C as well as other citrus plants and usually used as fruit for consumption. On the other hand, this plant has been used in medicine, such as sedatives for nervous disorders, cough spasms, hemorrhagic diseases, and epilepsy. It is said to be appetizing, cardiac stimulant, and antitoxic properties4. Citrus maxima fruit also contains high amounts of polyphenol compounds such as hesperidin, naringin, caffeic acid, p-Coumaric acid, ferulic acid, and vanillic acid3,5. Gas Chromatography-Mass Spectrophotometry (GCMS) is a chemical tool widely used to analyze GC-MS Analysis of Bioactive Compounds in Ethanol and Ethyl Acetate Fraction of Grapefruit (Citrus maxima L.) Rind