Polysaccharides Derived From the Brown Algae Lessonia nigrescens Enhance Salt Stress Tolerance to Wheat Seedlings by Enhancing the Antioxidant System and Modulating Intracellular Ion Concentration

Abstract

Soil salinity reduces plant growth and is a major factor that causes decreased agricultural productivity worldwide. Seaweed polysaccharides promote crop growth and improve plant resistance to abiotic stress. In this study, polysaccharides from brown seaweed Lessonia nigrescens polysaccharides (LNP) were extracted and further separated and fractionated. Two acidic polysaccharides (LNP-1 and LNP-2) from crude LNP were obtained and characterized. The latter had a lower molecular weight (MW) (40.2 kDa) than the former (63.9 kDa), but had higher uronic acid and sulfate content. Crude LNP and LNP-2 were composed of mannose, glucuronic acid, fucose, and xylose, whereas LNP-1 has little mannose. Moreover, the effects of the three polysaccharides on plant salt tolerance were investigated. The results showed that crude LNP, LNP-1, and LNP-2 promoted the growth of plants, decreased membrane lipid peroxidation, increased the chlorophyll content, improved antioxidant activities, and coordinated the efflux and compartmentation of intracellular ion. All three polysaccharides could induce plant resistance to salt stress, but LNP-2 was more effective than the other two. The present study allowed to conclude that both MW and sulfate degree contribute to salt resistance capability of polysaccharides derived from L. nigrescens.