No-Till and Solid Digestate Amendment Selectively Affect the Potential Denitrification Activity in Two Mediterranean Orchard Soils

Abstract

Improved soil managements that include reduced soil disturbance and organic amendment incorporation represent valuable strategies to counteract soil degradation processes that affect Mediterranean tree cultivations. However, changes induced by these practices can promote soil N loss through denitrification. Our research aimed to investigate the short-term effects of no-tillage and organic amendment with solid anaerobic digestate on the potential denitrification in two Mediterranean orchard soils showing contrasting properties in terms of texture and pH. Denitrifying enzyme activity (DEA) and selected soil variables (available C and N, microbial biomass C, basal respiration) were monitored in olive and orange tree orchard soils over a five-month period. Our results showed that the application of both practices increased soil DEA, with dynamics that varied according to the soil type. Increased bulk density, lowered soil aeration, and a promoting effect on soil microbial community growth were the main DEA triggers under no-tillage. Conversely, addition of digestate promoted DEA by increasing readily available C and N with a shorter effect in the olive grove soil, due to greater sorption and higher microbial efficiency, and a long-lasting consequence in the orange orchard soil related to a larger release of soluble substrates and their lower microbial use efficiency.