Molecular Characterization of Chelonid Alphaherpesvirus 5 in a Black Turtle (Chelonia mydas) Fibropapilloma from Baja California Sur, Mexico

Abstract

Simple Summary Fibropapillomatosis in sea turtles is a neoplastic disease associated with an infection by chelonid alphaherpesvirus 5, which can be fatal to turtles. The Baja California peninsula in the Mexican Pacific has been a relatively pristine environment for local aquatic wildlife; however, in the last decade, several turtles with this disease have been reported in the foraging areas of the region. Reasons for this are unknown but may be related to population growth, the surge of unchecked tourism, pollution, and fisheries, which have increased in the area over the past two decades. Finding a black turtle with fibropapillomatosis and chelonid alphaherpesvirus 5 in a natural protected area to host one of the most important foraging areas for sea turtles in the Mexican Pacific represents a potential risk for the population of black turtles and other species of sea turtles that visit the feeding grounds of the peninsula. This suggests a need to strengthen research lines on the west coast of Mexico and generate conservation strategies for organisms and the ecosystems that they inhabit. Abstract During routine monitoring in Ojo de Liebre Lagoon, Mexico, a juvenile black turtle (Chelonia mydas) was captured, physically examined, measured, weighed, sampled, and tagged. The turtle showed no clinical signs suggestive of disease. Eleven months later, this turtle was recaptured in the same area, during which one lesion suggestive of fibropapilloma on the neck was identified and sampled for histopathology and molecular analysis. Histopathology revealed hyperkeratosis, epidermal hyperplasia, acanthosis, papillary differentiation and ballooning degeneration of epidermal cells, increased fibroblasts in the dermis, and angiogenesis, among other things. Hematological values were similar to those reported for clinically healthy black turtles and did not show notable changes between the first capture and the recapture; likewise, clinicopathological evaluation did not show structural or functional damage in the turtle’s systems. The chelonid alphaherpesvirus 5 (ChHV5) UL30 gene was amplified and sequenced for phylogeny; Bayesian reconstruction showed a high alignment with the genus Scutavirus of the Eastern Pacific group. This is one of the first reports of ChHV5 in a cutaneous fibropapilloma of a black turtle in the Baja California peninsula.