Antibiotic-Resistant Bacteria in Green Turtle (Chelonia mydas) Rearing Seawater

Abstract

Simple Summary The Sea Turtle Conservation Center of Thailand (STCCT) has conducted an early intervention program for conservation and faced high mortality rates due to bacterial diseases. Our previous investigation of juvenile turtle carcasses and sea water in the turtle hold tanks implied an association between bacterial isolates in rearing water and infection in captive turtles. In this study, for a management plan of juvenile sea turtles with bacterial infection, we monitored antibiotic resistance of bacteria in seawater from juvenile green turtle holding tanks at STCCT in three periods: January 2015 to April 2016, January to April 2018, and January to April 2019. The results clearly indicated that numbers of resistant bacteria and antibiotics were increased. Assessment of resistance against ten antibiotics revealed high prevalence of antibiotic-resistant bacteria to the beta-lactam class (ampicillin, penicillin, and cefazolin), whereas low resistant isolate numbers were found to aminoglycosides. From the results of this study, we suggest that antibiotic-resistant bacterial assessment in sea turtle rearing seawater will provide important information for the treatment of bacteria-infected sea turtles in husbandry. Abstract Antibiotic resistance of microorganisms is a serious health problem for both humans and animals. Infection of these bacteria may result in therapy failure, leading to high mortality rates. During an early intervention program process, the Sea Turtle Conservation Center of Thailand (STCCT) has faced high mortality rates due to bacterial infection. Previously, investigation of juvenile turtle carcasses found etiological agents in tissue lesions. Further determination of sea water in the turtle holding tanks revealed a prevalence of these causative agents in water samples, implying association of bacterial isolates in rearing water and infection in captive turtles. In this study, we examined the antibiotic resistance of bacteria in seawater from the turtle holding tank for a management plan of juvenile turtles with bacterial infection. The examination was carried out in three periods: 2015 to 2016, 2018, and 2019. The highest isolate numbers were resistant to beta-lactam, whilst low aminoglycoside resistance rates were observed. No gentamicin-resistant isolate was detected. Seventy-nine isolates (71.17%) were resistant to at least one antibiotic. Consideration of resistant bacterial and antibiotic numbers over three sampling periods indicated increased risk of antibiotic-resistant bacteria to sea turtle health. Essentially, this study emphasizes the importance of antibiotic-resistant bacterial assessment in rearing seawater for sea turtle husbandry.