Effects of Dietary Aged Maize with Oxidized Fish Oil on Growth Performance, Antioxidant Capacity and Intestinal Health in Weaned Piglets

Abstract

Simple Summary In China, large quantities of maize are stored in grain depots for two years or more to mitigate the risk of natural disasters impacting feed supplies. However, it is unknown whether the use of long-term stored maize in diets will impair growth performance of piglets, and whether additional dietary oxidants would further exacerbate the effects. This study investigates the effects of dietary aged maize with the supplementation of different levels of oxidized fish oil on growth performance, nutrient digestibility, serum antioxidant activity and gut health in piglets and tries to provide a theoretical foundation for the better use of aged maize in swine production. The results of this study showed that aged maize had no significant effect on growth performance, diarrhea and nutrient digestibility of the piglets, but it did reduce serum antioxidant capacity. When oxidized fish oil was added, aged maize reduced serum antioxidant capacity further, inhibited the expressions of genes related to intestinal nutrient transport, promoted intestinal inflammation, and also reduced the apparent total tract digestibility (ATTD) of nutrients, increased diarrhea and finally reduced the growth performance of piglets. Thus, the use of aged maize in the diet of the piglets may be not feasible, especially when other oxidation-inducing factors existed, which would exacerbate the negative effects of the aged maize. Abstract This study aimed to determine the effects of dietary aged maize with supplementation of different levels of oxidized fish oil on growth performance, nutrient digestibility, serum antioxidant activity and gut health in piglets. Forty-two piglets were arranged in 2 × 3 factorial treatments in a complete randomized block design with seven replicates per treatment and one pig per replicate for 28 d. Diets included twp types of maize (normal maize or aged maize) and three levels of oxidized fish oil (OFO) (3% non-oxidized fish oil (0% OFO), 1.5% OFO and 1.5% non-oxidized fish oil (1.5% OFO), and 3% OFO (3% OFO). Results showed that dietary aged maize did not affect growth performance, diarrhea, and the apparent total tract digestibility (ATTD) of nutrients in piglets (p > 0.05). However, aged maize increased malonaldehyde (MDA) content and decreased total antioxidant capacity (T-AOC) in serum on both 14th and 28th days (p < 0.05) compared to the normal maize groups. Meanwhile, compared with normal maize, dietary aged maize showed a slight, but not significant (p > 0.10) decrease in total superoxide dismutase (T-SOD) activity and VE content in serum on the 14th day. In addition, aged maize significantly decreased GLUT2 mRNA expression (p < 0.05) and tended to increase (p < 0.10) TNF-α and IL-6 mRNA expression in jejunal mucosa. Compared with non-oxidized fish oil, oxidized fish oil resulted in the decrease of the 14–28 d and 0–28 d ADG, as well as the ATTD of dry matter (DM), ether extract (EE), organic matter (OM) (p < 0.05), whereas the increase in diarrhea index (p < 0.05) and F/G of the whole period (p < 0.05). Oxidized fish oil decreased serum T-AOC on both the 14th and the 28th days (p < 0.05), and decreased serum T-SOD activity and VE content on the 28th day (p < 0.05), whereas increased serum MDA content on the 28th day (p < 0.05) and 14th day (p < 0.10) compared with fresh fish oil. Meanwhile, MUC2 (p < 0.05) and SGLT1 (p < 0.10) mRNA expression in jejunal mucosa were decreased compared with non-oxidized fish oil. In addition, dietary oxidized fish oil tended to decrease 14–28 d ADFI and the ATTD of CP (p < 0.10), and piglets fed oxidized fish oil significantly decreased 14–28 d ADFI, the ATTD of CP, GLUT2 and SGLT1 mRNA expressions in jejunal mucosa when piglet also fed with aged maize (p < 0.05). Collectively, these results indicated that dietary oxidized fish oil decreased growth performance and nutrients digestibility of piglets fed with aged maize. This nutrient interaction may be mediated by inhibiting intestinal nutrient transporter, inducing intestinal inflammation, and reducing antioxidant capacity.