Comparison of different STR Typing for a Special Allele at D7S820 Locus by Using Ten Different STR Multiplex System

Abstract

Nowadays, more and more rare alleles are studied and supplemented to common Loci system, especially for the construction of DNA database and population studies. The ability to accurately judge the rare Loci become more essential. D7S820 is one of the useful markers for human identification, paternity and maternity testing and sex determination in forensic sciences [1]. It has been revealed 4 microvariant alleles: 8.1, 9.1, 10.1 and 10.3 [2]. Here we designed a study to evaluate the performance of allele typing at D7S820 locus, with ten different STR multiplex systems, which mostly have been used in current forensic application for a long time. In this paper, we observed an abnormal genotype shown different results when tested by ten different STR multiplex kits.