Assessment antiviral activity of functional textiles: pioneering service offered by Bio- Manguinhos to partners

Abstract

Methodology: To meet this demand, it was designed a work plan, outlined a pricing strategy and developed a technical protocol adapted from ISO18184. Textile samples were analyzed at different steps: analysis of cytotoxicity in NB-2 and antiviral activity against the Measles virus (previous antiviral efficacy screening in NB-2) and/or against the SARS-CoV-2 virus in NB-3. Briefly, after cytotoxicity analysis approved, samples (20mm x 20mm) of control or formulated textiles were challenged with a virus suspension for 1 or 30 minutes (contact time/room temperature = Fabric plus Virus). Afterwards, washed out samples with recovered viruses were quantified by TCID50 method, using Vero CCL-81 cells to determine the virus infectivity titer. Likewise, antiviral samples submitted industrially washed were evaluated for wash-stable. The antiviral performance of products were measured and the differences among 2.0-3.0 (log TDID50/mL) or higher than 3.0 (log TDID50/mL) were considered as good or excellent effect, with antiviral efficacy higher than 99% or 99.9%, respectively.