IN VITRO CYTOTOXICITY EVALUATION OF NOVEL COMBINED PLANT CRUDE AQUEOUS EXTRACT OF LICORICE AND MACA

Abstract

Recently, researchers have shown an increased interest in the potential positive effects of Glycyrrhiza glabra (licorice) and Maca extracts. However, there has been no detailed investigation of the effects of mixing these two plants extracts. Therefore, the present research explores for the first time, the effects of the mixture of these two extracts. The aim of the current study is to figure out the cytotoxicity of this mixture on the sensitive system of the mammal s body. This study, therefore, set out to assess the cytotoxicity effect of the aqueous crude extract of Licorice, Maca, and the effect of their extracts mixture on mice normal spleenocytes by MTT assay. The results show that the cells growth inhibition of both extracts and their mixture was 0% compare to control. The mixture medium (12.5/2.5mg) has the best viability with 245.0% compared to Licorice medium alone (12.5mg) with viability 223.1% and Maca medium alone (2.5mg) with viability 133.6 %. The most obvious finding to emerge from this study is that the mixtures of licorice and Maca extracts have no harmful effects at a dose of (12.5/2.5mg). Furthermore, the mixture data can be applied for orally used in the treatment of infertility status.