Annals of Tropical Medicine and Public Health | 2019
Transcription Factor 7-Like 2 Gene Polymorphisms rs7903146associationwith Type 2 Diabetic Polycystic Ovarian Syndrome Women of Iraqi Population
Abstract
Background: TCF7L2(rs7903146) gene product is a high mobility group box-containing transcription factor implicated in blood glucose homeostasis. Polycystic ovary syndromeis a prevalent reproductive and metabolic disorder with variable phenotypes and an underlying pathophysiology that is still not completely understood. The clinical presentation of polycystic ovary syndrome is widely variable, with complaints encompassing oligomenorrhoea, infertility, obesity, hirsutism, endometrial cancer, and diabetes. The aim of current study was to investigatethe association between Transcription Factor 7-Like 2 Gene(rs7903146) polymorphisms with Type 2 diabetic with polycystic ovarian syndrome Women of Iraqi Population. Methods: This study was conducted on 150subjects(50 patients with polycystic ovary syndrome, 50 diabetic patients and 50 healthy individuals). Blood glucose concentration and HbA1c were measured in spectral methods using enzymatic measures, C-peptide, insulin measurement by ELISA and measurement of FSH, prolactin and LH by Manifides. The gene rs7903146 (TCF7L2) was described by PCR-ARMS. Results: There was significant elevation of T allele in diabetic polycystic ovarian syndrome compared with controls (32% versus 14%, respectively). Both CT and TT genotypes were significantly increased in patients compared with controls and increased risk (OR 2.2, OR 2.6respectively) and polycystic ovarian syndrome risk with CT genotype (OR 2.6 ). An association was found among CT, TT genotypes, hormonal and biochemical traits in patients. An association was found between two genotypes (CT and TT) with the occurrence of both diabetic polycystic ovarian syndrome and polycystic ovarian syndrome in the Iraqi women. Conclusion: TCF7L2 is associated with T2DM in this Iraqi population. The association is not dependent on a specific T2DM phenotype. Clinical genetic testing for TCF7L2 can help predict the occurrence of T2DM in Iraqi women.